Jesse joins the The Treblemakers and later becomes the lead singer when Bumper ditches the group to sing backup for John Mayer. After the 2012 ICCA Finals, Jesse is revealed to have become the leader of the Treblemakers, appearing at that year's a cappella auditions.
Freshly graduated from high school, Jesse enrolls at Barden University, where he auditions for the Barden Treblemakers, and subsequently gets in. Jesse spends most of the movie trying to get close to Beca Mitchell.
He also always looks out for her, although she eventually snaps and tells him to back off and stop. Utterly shattered, he completely breaks down, going into a depression, rarely leaving his room and becoming visibly unkempt.
Beca comes to apologize, and Jesse initially rebuffs her, telling her to "figure it out." At the finals, Jesse becomes the leader of the Treblemakers, retaining his position as lead singer, along with Benji Applebaum. As the Bellas sing Beca's set list, they sing the theme song to The Breakfast Club, and Beca and Jesse reconcile their differences with a kiss.
He is currently still in a relationship with Beca and is still the leader of the Treblemakers.
Jesse is friendly and very outgoing, displaying a very jovial, extroverted nature. He quickly befriends his socially awkward roommate, Benji Applebaum, and is quick to be friends with Beca. Jesse is very loyal and a passionate singer, eventually auditioning and getting into the Barden Treblemakers.
His loyalty becomes apparent as Jesse backs up Beca whenever she gets into trouble, though she seems to be more annoyed than grateful for this. Jesse, though, knows when to take the hint. After Beca snaps at Jesse, saying that she doesn't need his help, he starts to back off, though they eventually reconcile and become a couple.
Jesse is also an avid movie fan, his favorite movies are Jaws, Million Dollar Baby, The Breakfast Club, Rocky and E.T. the Extra-Terrestrial.
Beca is Jesse's close friend who later on became his girlfriend. They share a good relationship together, as Jesse's always looking out for her, and comforts Beca when she's down. In Pitch Perfect 2, he showed his support to Beca when she entered an internship at a recording studio, at the Acapella World Championship Competition and still very much in love.
Skylar Astin portrays Jesse in the film Pitch Perfect. Skylar has a sister named Brielle and two brothers.
- Jesse's actor, Skylar Astin, voiced Roy, a Nicelander in the movie Wreck-It Ralph.
- Skylar Astin is engaged to Anna Camp, who plays Aubrey Posen.
- He and Benji are the new leaders of the Treblemakers.